The correct option is 4: Tissue culture. Somaclonal variation is, by its definitive biological concept, the genetic and phenotypic variability naturally generated among plants regenerated from any form of plant cell or tissue culture. This phenomenon of variation occurs spontaneously during the in vitro (in glass) propagation process, especially when plants are regenerated from a disorganized mass of cells called callus, but it can also arise from shoot tip culture.…Read More