Question
Easy
Which one of the following is not required during Polymerase Chain Reaction (PCR)?
1
DNA template
2
Chitinase enzyme
3
Nucleotide primers
4
DNA polymerase enzyme
Question Details
Time to Solve: 12
Exam: HTET
Level/Paper: HTET_L3
Chapter:
Topic:
Correct Answer
Option B
Explanation
The correct option is 2: Chitinase enzyme The Polymerase Chain Reaction (PCR) is a revolutionary technique in molecular biology used to rapidly amplify a specific segment of DNA into millions or billions of copies. The process itself is an in vitro replication cycle requiring four fundamental components: a DNA template, oligonucleotide primers, a heat-stable DNA polymerase (like Taq polymerase), and deoxyribonucleotides (dNTPs). Chitinase enzyme, by contrast, plays no direct role…Read More
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